STANDARDIZATION IN VITRO ANTIOXIDANT ACTIVITY AND BRINE SHRIMP LETHALITY OF A POLYHERBAL FORMULATION

*Macha Vijay; K Ramakanth Reddy; M Ravi Kumar Reddy; K Gnananath; B Surendra Babu

*Macha Vijay Source Natural Foods and Herbal Supplements Limited Plot No 22 & 23, SVCIE, Bachupally, Quthbullapur, RR District
K Ramakanth Reddy Source Natural Foods and Herbal Supplements Limited Plot No 22 & 23, SVCIE, Bachupally, Quthbullapur, RR District
M Ravi Kumar Reddy Source Natural Foods and Herbal Supplements Limited Plot No 22 & 23, SVCIE, Bachupally, Quthbullapur, RR District
K Gnananath Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram, West Godavari District, Andhra Pradesh
B Surendra Babu Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram, West Godavari District, Andhra Pradesh

Keywords: Shakti drops, Antimicrobial, Antioxidant, Standardization, cytotoxicity.

Abstract

Shakti drops an Ayurvedic polyherbal formulation is an effective medicine for immuno modulatory activity. The formulation was prepared in the laboratory with authenticated organic plant drugs. The standardization procedure was conducted on the basis of physicochemical parameters and antimicrobial studies as directed by Ayurvedic formulary of India. Antioxidant activity is further performed based on its prescribed dose in three In-vitro antioxidant models. Physical characters remained unaltered throughout study, In antimicrobial activity total aerobic count was found to be 18Ã—10¹ CFU/mL and total fungal count was found to be <10 CFU/mL. It is devoid of all tested microorganism i.e. Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli tested. In antioxidant activity the results of the study suggest that the Shiakti drops has potent antioxidant and free radical scavenging activity justifying its use in Rasayana.