ESTIMATION OF TOTAL PHENOLIC AND FLAVONOID CONTENT OF POLYHERBAL FORMULATION DASHANG LEPA

Soni Ashish; S J Gupta; Kumar Amit; Y B Tripathi

Soni Ashish Consultant, Anorectal Surgeon, Anand Hospital Beawer, Rajasthan, India.
S J Gupta Assistant Professor, Department of Shalya Tantra, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Kumar Amit Ph.D scholar, Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Y B Tripathi Professor, Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.

Keywords: Polyherbal compound, Dashang lepa, Phenolic, Flavonoid, Follin ciocalteau reagent, quercetin.

Abstract

Background: Indian system of medicine mainly based on polyherbal compound to treat various types of diseases. Dashang lepa is one of the prestigious formulation of ten indigenous drugs which are having predominantly anti-inflammatory properties and clinically used in inflammatory swelling like boil, furunculosis, cellulitis, erysipelas etc. As several reports are on various individual phytochemical, phenolic and flavonoid content but none is having study of polyherbal compound Dashang lepa. Objectives: Total phenolic and total flavonoid content were studied of alcoholic extract of Dashang lepa. Method: Methanolic extraction was done by hot percolation method through soxhlet apparatus. Phenolic content was estimated using Follin ciocalteau reagent, flavonoid using aluminum chloride (2%) reagent as quercetin equivalent. Results: Estimation of total phenolic and total flavonoid content showed that Dashang lepa extract was having phenolic content (15.8±1.14) ug Gallic acid equivalents (GAE) and flavonoid content (22.4±2.54) in ug of quercetin equivalents (QE).